Effects of dietary garlic (Allium sativum) oil on growth performance, haemato-biochemical and histopathology of cypermethrin-intoxicated Nile tilapia (Oreochromis niloticus).

BACKGROUND: When pesticides are introduced into wetlands by agriculture, fish quickly absorb them through their gills. Pesticides reduce hatchability, impede growth, and antioxidant response, killing fish. Therefore, it's crucial to find effective pesticide mitigation methods for fish. OBJECTIVE: In this study, the effects of garlic (Allium sativum) oil on the growth, haematology, biochemistry and histopathology parameters of Nile tilapia (Oreochromis niloticus) exposed to cypermethrin toxicity were investigated. METHODS: In the research, cypermethrin was added to the water of the experimental groups at a rate of 1:20 of the LC50 value, and 1.00% garlic oil was added to the fish feed. Fish with an initial weight of 30.26 ± 0.26 g were fed for 45 days. RESULTS: At the end of feeding, the final weights were determined as 69.39 ± 0.41 (G1), 61.81 ± 0.65 (G2), 82.25 ± 0.36 (G3), and 75.04 ± 0.68 (G4) grams, respectively. Histopathological examinations revealed serious lesions in the gill, liver, brain, and muscle tissues in the cypermethrin group, whereas these lesions were minimal or absent in the garlic oil group. CONCLUSIONS: Garlic oil supplementation had positive effects on growth, haematology, blood biochemistry, hepatosomatic index and histopathological parameters. These findings suggest that garlic oil is a potential protective agent against cypermethrin toxicity.

Effects of glutamine on growth performance, nutrient content, fatty acid profile, and blood parameters of rainbow trout (Oncorhynchus mykiss).

In this study, different amounts of glutamine were added to the diet of rainbow trout, and they were then fed for a period of 90 days. The current research investigated the effects of glutamine on various aspects of rainbow trout, including growth performance, condition factor, viscerosomatic index, hepatosomatic index, carcass composition, fatty acid profile, hematological parameters, and biochemical parameters. The study's findings revealed that adding glutamine to the diet of rainbow trout had a beneficial impact on their growth features. The rainbow trout group that was fed a 2% concentration of glutamine experienced the most notable increase in growth rate. A statistically significant difference in growth was observed among all groups (p < 0.05). Adding glutamine to the diet increased the amount of protein and decreased the fat content in the flesh of the fish. Glutamine exerted an influence on the blood and biochemistry parameters of fish, as well as their fatty acid composition. In conclusion, the inclusion of glutamine in the diet of fish could potentially enhance their immune system, improve the quality of their muscles, and enhance their growth performance.

Effect of garlic (Allium sativum) - supplemented diet on growth performance, body composition and fatty acid profile of rainbow trout (Oncorhynchus mykiss).

In this study, rainbow trout were fed for a total of 90 days with different feed mixes containing 0.00%, 1.00%, 1.50% and 2.00% garlic (Allium sativum). The effect of garlic on the growth performance, body composition and fatty acid profile of rainbow trout was researched. The starting weight of fish in the experimental group was approximately 64.12±0.29 gr, which increased to 234.12±3.54gr, 246.31±4.41gr, 258.74±4.42gr, and 268.79±4.79gr, while the difference in growth between the groups was observed to be statistically significant (p<0.05). At the end of the feeding period, the specific growth rate (SGR), the economic conversion ratio (ECR), the feed conversion ratio (FCR), the economic profit index (EPI) and the protein efficiency ratio (PER) were checked. In this study, the nutritional composition and fatty acid profiles of fish after 90 days of feeding were examined. As a result of this study, the supplementation of garlic to the diet given to rainbow trout caused an increase in their protein ratio and a decrease in their moisture content and fat ratio. Additionally, it led to a decrease in fatty acid and monounsaturated fatty acid levels and an increase in polyunsaturated fatty acids.

Silent threat in honey bee colonies: infection dynamics and molecular epidemiological assessment of black queen cell virus in Turkey.

Viruses can have devastating effects and cause epidemics in honey bee (Apis mellifera) colonies. Black queen cell virus (BQCV), which is one of the most common honey bee viruses, affects queen bee larvae and their pupae. This study provides information on the dynamics of BQCV infection in honey bees, using molecular diagnostics to investigate the effects of other pathogens and seasonal patterns that are considered relevant to the epidemiology of BQCV. The results showed a relatively high prevalence of the viruses studied. The prevalence of BQCV, acute bee paralysis virus, and deformed wing virus in worker bees was found to be 90%, 62%, and 84%, respectively. The prevalence of BQCV was 58% in larvae and pupae. Furthermore, the prevalence of Nosema ceranae was 46% in worker bees. Statistical analysis showed possible combined effects of BQCV and other examined viruses in terms of infection dynamics, while BQCV did not show seasonal variation. The BQCV isolates detected in this study were placed in a phylogenetic framework using sequence data from comprehensive sampling in previous studies. The analysis suggested that the Turkish strains of BQCV clustered together with Australian and European strains and consisted of homogeneous populations that had evolved from a common ancestor. This is the first report of BQCV infection dynamics in honey bees in Turkey.

A high-resolution melting assay to differentiate a peste des petits ruminants virus vaccine strain from field isolates in Turkey.

Peste des petits ruminants (PPR) mostly affects small ruminants. Vaccination with attenuated vaccines derived from PPR virus (PPRV) provides successful protection against the disease. However, current molecular methods are unable to distinguish vaccine strains from field strains. In this study, we used an EvaGreen-based high-resolution melting (HRM) assay to differentiate a PPRV vaccine strain (Nigeria 75/1) from Turkish field isolates of lineage IV based on melting peaks and melting profiles.

Effects of boric acid on the growth performance and nutritional content of rainbow trout (Oncorhynchus mykiss).

In this study, boric acid was added to the rainbow trout (Oncorhynchus mykiss) feed at ratios of 0.00%, 0.01%, 0.05%, 0.10%, and 0.20%. The feeding period of this study continued for 90 days. The effect of boric acid on the growth parameters and nutritional composition of rainbow trout were investigated. In this research, effects on live weight gain (LWG), amount of feed intake, feed conversion rate (FCR), protein efficiency rate (PER), specific growth rate (SGR), survival rate, economic conversion rate (ECR), and economic profit index (EPI) were evaluated as growth parameters. As for nutritional contents, the total crude protein, lipid, raw ash, and humidity ratios were calculated. Rainbow trout with an initial weight of 92.04 g reached up to 195.05 ± 1.69 g, 202.69 ± 1.94 g, 217.53 ± 2.84 g, 195.25 ± 2.18 g, and 181.20 ± 1.89 g, respectively in the different levels of boric acid at the end of the trial period. The best growth performance was obtained in the group with 0.05% boric acid added, while the lowest growth performance was observed in the group with 0.20% boric acid added. It was concluded based on the results of this study that the amount of boric acid up to 0.05% in the fish feed positively affected the growth parameters and the addition of higher amounts had either no benefit or negative effects on the growth performance of rainbow trout. Furthermore, boric acid in feed affected the nutritional composition of fish meat.

Histopathology changes in the rainbow trout (Onchorhyncus mykiss) consuming boric acid supplemented fish fodder.

OBJECTIVE: Even though boron (B), as a trace micronutrient, occurs in natural waters and organisms, its high concentration could cause harmful and even toxic for organisms. The aim of present study was to investigate the effects of boric acid (BA) added to feed (0.01%, 0.05%, 0.10%, and 0.20% of B in feed) on Rainbow Trout (Oncorhynchus mykiss) by histopathological methods and compared to the control feed as without B. METHODOLOGY: At the end of the feeding, after weighing the live weight of 9 rainbow trout from each group, tissue fragments were taken from the liver, gill, kidney, skeletal muscle, spleen and brains of fish, which systemic necropsies was done, and were fixed in 10 % buffered formalin solution. RESULTS: Histopathological examinations revealed degenerative and necrotic changes in the liver, gill, kidney, skeletal muscle, spleen and brain. Hydropic and vacuolar degenerations in liver parenchyma, lamellar edema in the gills, hyaline accumulation in the kidneys, degenerations in the muscles, necrosis in the spleen and hyperemia in the brain were observed in all groups except control group. It was observed that the destruction of boric acid on fish increased depending on the amount of boron supplemented to the feed, and the most effect was in the group fed with 0.20 % boron supplemented feed. CONCLUSIONS: The findings aside from causing pathological changes in all organs in terms of histopathological findings of Boric Acid (BA), the most severe lesions were observed in the liver.

Detection of Peste des petits ruminants virus RNA in Culicoides imicola (Diptera: Ceratopogonidae) in Turkey.

An outbreak of Peste des petits ruminants (PPR) occurred in the Antalya Province in Turkey during  October  2015.  The  Antalya  Province  has  suitable  habitats  for  vectors.  There  is  no  information  available  on  the  role  of  Culicoides  spp.  in  the  transmission  of  Peste  des  petits  ruminants virus (PPRV). In this study we investigated the potential role of the Culicoides spp. in  the  transmission  of  PPRV.  Culicoides  were  trapped  throughout  middle  of  October  and  middle of December, 2015. A total of 12 pools of non-engorged females were analysed with real-time RT-PCR targeting the nucleocapsid (N) gene of the PPRV. PPRV RNA was detected in 7 of 12 Culicoides pools. These pools were negative for the bovine/ovine beta-actin mRNA. Culicoides spp. were identified to the species level by sequence analysis of the mitochondrial cytochrome  oxidase  subunit  I  gene.  The  species  of  Culicoides  found  PPRV  positive  was  Culicoides  imicola.  Molecular  characterization  of  field  isolates  from  recent  outbreaks  and  pools of midges that tested positive for PPRV suggests that PPRV replication might occur in Culicoides imicola, and it may have played a role in transmitting PPRV.

Current stretched penile length: cross-sectional study of 1040 healthy Turkish children aged 0 to 5 years.

OBJECTIVES: To update the normal stretched penile length values for children younger than 5 years of age. We also evaluated the association between penile length and anthropometric measures such as body weight, height, and body mass index. METHODS: The study was performed as a cross-section study. The stretched penile lengths of 1040 white uncircumcised male infants and children 0 to 5 years of age were measured, and the mean length for each age group and the rate of increase in penile length were calculated. The correlation between penile length and weight, height, and body mass index of the children was determined by Pearson analysis. RESULTS: The stretched penile length was 3.65 +/- 0.27 cm in full-term newborns (n = 165) and 3.95 +/- 0.35 cm in children 1 to 3 months old (n = 112), 4.26 +/- 0.40 cm in those 3.1 to 6 months old (n = 130), 4.65 +/- 0.47 cm in those 6.1 to 12 months old (n = 148), 4.82 +/- 0.44 cm in those 12.1 to 24 months old (n = 135), 5.15 +/- 0.46 cm in those 24.1 to 36 months old (n = 120), 5.58 +/- 0.47 cm in those 36.1 to 48 months old (n = 117), and 6.02 +/- 0.50 cm in those 48.1 to 60 months old (n = 113). The fastest rate of increase in penile length was seen in the first 6 months of age, with a value of 1 mm/mo. A significant correlation was found between penile length and the weight, height, and body mass index of the boys (r = 0.881, r = 0.864, and r = 0.173, respectively; P = 0.001). CONCLUSIONS: The age-related values of penile length must be known to be able to determine abnormal penile sizes and to monitor treatment of underlying diseases. Our study has provided updated reference values for penile lengths for Turkish and other white boys aged 0 to 5 years.